Utilize este identificador para referenciar este registo: http://hdl.handle.net/10437/12146
Título: Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides
Autores: Ribeiro, Geise Camila de Araújo
Fernandes, Pedro Carlos de Barros
Silva, Dayse Alessandra Almeida
Brandão, Hugo Neves
Assis, Sandra Aparecida de
Palavras-chave: BIOTECNOLOGIA
BIOQUÍMICA
BIOQUÍMICA ALIMENTAR
BIOTECHNOLOGY
BIOCHEMISTRY
FOOD BIOCHEMISTRY
Data: 2021
Editora: Springer
Resumo: The crude extract containing inulinase from Rhodotorula mucilaginosa was obtained by submerged fermentation. Inulinase was immobilized on chicken eggshell by physical adsorption and covalent crosslinking, using glutaraldehyde as a crosslinking reagent, and Celite by adsorption. Fructooligosaccharides production was performed using immobilized inulinase (5%, w/v) and inulin substrate solution under experimental conditions evaluated through Doehlert experimental design. The production of inulinase was optimized for concentrations of D-glucose and yeast extract at 12.5 and 0.5 g/L, respectively, resulting in an optimal activity of 0.62 U. The optimal pH and temperature for enzyme activity were 8.0 and 75 °C, respectively, leading to an optimal activity of 3.54 U. The highest immobilization efficiency (46.27%) was obtained upon immobilization on Celite. Immobilization by adsorption to eggshell allowed for specific activity of 4.15 U/g, and adsorption to Celite resulted in specific activity of 3.70 U/g. The highest titer in fructooligosaccharides was obtained with an initial inulin concentration of 250 g/L (25%, w/v), and a reaction time of 16 h. Hence, immobilized inulinase proved to be a promising catalyst for fructooligosaccharides production since the formulation is performed through a simple, low-cost, and large-scale applicable methodology.
Descrição: Food Science and Biotechnology
URI: http://hdl.handle.net/10437/12146
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