Quantifying hepatic fibrosis on murine models: how to obtain representative results in a less laborious way

DSpace/Manakin Repository

Quantifying hepatic fibrosis on murine models: how to obtain representative results in a less laborious way

Show full item record

Title: Quantifying hepatic fibrosis on murine models: how to obtain representative results in a less laborious way
Author: Catarino, José; Viegas, Inês; Rodrigues, Joana; Pinto, Marta; Faísca, Pedro
Abstract: In biomedical research, quantification of histological images is often required. Many of the methods used are time-consuming, laborious, originate variable results and are difficult to replicate. This paper is aimed towards finding a more objective, reproducible and easy to perform method to obtain representative results in murine models of hepatic fibrosis using ImageJ software. To do so on a liver fibrosis model, the percentages of fibrotic lesion obtained in an entire section and in several different magnifications were compared. No statistically significant differences were found (p> 0.05), but the correlation was stronger between the results obtained in the entire section photograph and the two photographs at 40x (r = 0.963). Using ImageJ facilitated the definition of a methodology that originated representative results on a liver section and at the same time allowed objective measurement in a reproducible and less laborious way.
Description: Revista Lusófona de Ciência e Medicina Veterinária
URI: http://hdl.handle.net/10437/9492
Date: 2019


Files in this item

Files Size Format View Description
6658-385-19797-1-10-20190218.pdf 338.3Kb PDF View/Open Artigo

This item appears in the following Collection(s)

Show full item record

Search DSpace


Advanced Search

Browse

My Account